<p>&nbsp;</p> <div style="margin: 2pt 0in 4.8pt">S-Nitrosothiols (RSNO) are metabolites of nitric oxide that have been implicated to participate in NO signaling pathways. RSNO can form on both low-molecular weight thiols (such as cysteine and glutathione) or on protein thiols and can exchange between thiol groups by a process referred to as transnitrosation. Despite a growing literature on the control of cellular signaling via S-nitrosation, the mechanisms by which NO transmits its signals through thiol groups are poorly understood. BY making use of the fact that S-nitrosocysteine is specifically transported into cells via amino-acid transport system L, we have investigated mechanisms of NO and RSNO-dependent protein modification and found that they can vary considerably. In addition, we have examined using novel proteomic methods the level and distribution of S-nitrosation at close to physiological levels of S-nitrosothiol. Finally we have investigated novel mechanisms of S-nitrosothiol synthesis. In total we are attempting to put a mechanistic framework behind the process of protein S-nitrosation within the context of the cellular environment.</div> Medical College of Wisconsin 2009-09-10T18:30:12-07:00 Xian 77 Fulmer 438 Prof. Neil Hogg 2009-11-09T16:10:00-08:00 2009-10-29T14:11:11-07:00